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  • During in vitro synthesis of DNA, a researcher used 2', 3' – dideoxy cytidine triphosphate as raw nucleotide in place of 2'-deoxycytidine. What would be the consequence?

During in vitro synthesis of DNA, a researcher used 2', 3' – dideoxy cytidine triphosphate as raw nucleotide in place of 2'-deoxycytidine. What would be the consequence?

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2', 3' - dideoxy cytidine triphosphate is a reverse transcriptase inhibitor. Reverse transcriptase Is a viral DNA polymerase which facilitates DNA replication In HIV and other retroviruses. The commercial name of ddC is Zalcitabine, and it is sold as a pharmaceutical product for the management of HIV. If 2', 3'- dideoxy cytidine triphosphate is used as a raw nucleotide in place of 2' - deoxycytidine, it will stop DNA replication. The researcher will not be able to proceed on his or her experiment because of the contrary effect of his or her chosen reagent.

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