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ELISA technique is based on the principles of antigen-antibody interaction. Can this technique be used in the molecular diagnosis of a genetic disorder, such as phenylketonuria?
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Yes. One can use an antibody against the enzyme (that is responsible for the metabolism of phenylalanine) to develop ELISA based is a diagnostic technique. The patient where the enzyme protein is absent would give a negative result in ELISA when compared to a normal individual.
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