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  • Replication was allowed to take place in the presence of radioactive deoxynucleotides precursors in E. coli that was a mutant for DNA ligase. Newly synthesised radioactive DNA was purified, and strands were separated by denaturation.

Replication was allowed to take place in the presence of radioactive deoxynucleotides precursors in E. coli that was a mutant for DNA ligase. Newly synthesised radioactive DNA was purified, and strands were separated by denaturation. These were centrifuged using density gradient centrifugation. Which of the following would be a correct result?

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Let us assume that heavier nitrogen was used in this experiment. This nitrogen molecule from parent's cell would be transmitted equally in the daughter cells. Each daughter cell will have half of the DNA with heavier nitrogen and another half with lighter nitrogen.

In the F2 generation, 50% of daughter cells will have a combination of radioactive and non-radioactive DNAs. The rest 50% of daughter cells will have non-radioactive DNAs.

This is the reason why the graph shows two peaks with each peak representing a particular form of nitrogen in DNA.

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