To isolate protoplast, one needs:
a. pectinase
b. cellulase
c. both pectinase and cellulase
d. chitinase
The answer is option (c) both pectinase and cellulase.
A Protoplast is a plant cell without a cell wall. The cell wall is composed of pectin and cellulose. Therefore, to digest those substances, digestive enzymes like pectinase and cellulase are used.