Discuss briefly how pure lines are created in animal husbandry.
Pure lines are created using in-breeding. When breeding is done with closely related individuals, it is called inbreeding. A superior male and a superior female are selected for this purpose, and they are mated. From the next generation, superior male and female are selected to continue the process. This is continued for 4 to 5 generations to achieve pure line. Inbreeding increases homozygosity and thus enables to obtain pure lines. However, inbreeding depression is a major problem with this method.